Northwestern University experts published to Nature “Mega-scale experimental analysis of protein folding stability in biology and design” in July 2023. The research team utilized EME’s cnvK linker for development of cDNA display proteolysis method.
Northwestern University experts; Dr. Kotaro Tsuboyama (Institute of Industrial Science, The University of Tokyo, Japan) and Dr. Gabriel Rocklin (Assistant professor of Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA) published to Nature “Mega-scale experimental analysis of protein folding stability in biology and design” in July 2023. In this research, the research team developed cDNA display proteolysis, a method for the parallel measurement of thermodynamic folding stability for up to 900,000 protein domains in a one-week experiment. This research implies the ability of inventing machine learning for identifying specific amino acid mutations which cause diseases and synthesizing draggable domains of drug discovery.
cDNA display *1 is one of the most important key technologies for cDNA display proteolysis method, and the researchers utilized cnvK linker *2 (Epsilon Molecular Engineering, Inc.) for synthesizing cDNA display molecules. Our cnvK linker is a very unique linker, and it allows synthesizing nearly a million of highly-stable cDNA display molecules. EME was really delighted that our cnvK linker contributed greatly to the success of this research.
*1. cDNA display: Most stable and diverse genotype-phenotype coupling using cell-free
translation system, enabling 10 13-14 repertories of VHHs to be screened at once.
*2. cnvK linker: For cDNA display method, the puromycin linker is the key technology of
genotype-phenotype linking strategy. As this puromycin linker, EME uses our unique
puromycin linker which contains 3-cyanovinylcarbazole nucleoside (cnvK), and it is called
Tsuboyama, K., Dauparas, J., Chen, J., Laine, E., Mohseni Behbahani, Y., Weinstein, J. J., Mangan, N. M., Ovchinnikov, S., & Rocklin, G. J. (2023). Mega-scale experimental analysis
of protein folding stability in biology and design. Nature. https://doi.org/10.1038/s41586-023-06328-6
Press release (Tokyo University): New method expertly evaluates protein folding stability on a large scale