論文 / 特許

論文

2019年

Anzai H, Terai T, Jayathilake C, Suzuki T, Nemoto N., A novel immuno-PCR method using cDNA display. Analytical Biochemistry, 578, 1-6 (2019)

2018年

Nemoto N, Kumachi S, Arai H., In vitro Selection of Single-Domain Antibody (VHH) using cDNA display, "Antibody Engineering 3rd Edition (edited by D. Nevoltis & P. Chames)", Methods in Mol. Biol., 1827, 269-285 (2018)

Suzuki T, Nochizuki Y, Kimura S, Akazawa-Ogawa Y, Hagihara Y, Nemoto N., Anti-survivin single-domain antibodies derived from an artificial library including three synthetic random regions by in vitro selection using cDNA display, Biochem. Biophys. Res. Commun., 503, 3, 2054-2060 (2018)

2017年

寺井琢也, 熊地重文, 根本直人, 「cDNAディスプレイ法によるペプチドスクリーニング技術」, 技術情報協会「ペプチド医薬品のスクリーニング・安定化・製剤化技術」第1章, 第6節, 40-49 (2017)

Kobayashi S, Terai T, Yoshikawa Y, Ohkawa R, Edihara M, Hayashi M, Takiguchi K, Nemoto N., In vitro selection of random peptides against artificial lipid bilayers: a potential tool to immobilize molecules on membranes, Chem. Commun. (Camb.), 53, 3458-3461 (2017)

2016年

熊地重文, 根本直人, 「試験管内進化を加速するcDNA displayシステム」酵素工学ニュース, 76, 21-25 (2016)

根本直人, 「cDNAディスプレイによる機能性ペプチドアプタマーの創生」 日本生物工学会誌, 94, 481-484 (2016)

Kumachi S, Husimi Y, Nemoto N., An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display. ACS Omega. 1, 52-57 (2016)

2015年

Mochizuki Y, Suzuki T, Fujimoto K, Nemoto N., A Versatile puromycin-linker using cnvK for high-throughput in vitro selection by cDNA display. J. Biotechnol. 212, 174-180 (2015)

Tanemura Y, Mochizuki Y, Kumachi S, Nemoto N., Easy and rapid binding assay for fanctional analysis of disulfide-containing peptides by a pull-down method using a puromycin-linker and a cell-free translation system, Biology (Basel), 4, 161-172 (2015)

2014年

Nemoto N, Fukushima T, Kumachi S, Suzuki M, Nishigaki K, kubo T., Versatile C-terminal specific biotinylation of proteins using both a puromycin-linker and a cell-free translation system for studying high-throughput protein-molecule interations, Anal. Chem., 86, 8535-8540 (2014)

Mochizuki Y, Nisigaki K, Nemoto N., Amino group binding peptide aptamers with double disulphide-bridged loops selected by in vitro selection using cDNA display., Chem. Commun. (Camb.), 50, 5608-5610 (2014)

2013年

根本直人, 「遺伝子型-表現型対応付け技術」 (株)エヌ・ティー・エス 『進化分子工学-高速分子進化によるタンパク質・核酸の開発-』第2編, 第3章, 161-170 (2013)

    

根本直人, 望月佑樹, 上野真吾 「cDNA displayによる分子デザイン -mRNA display(in vitro virus)からcDNA displayへ-」 生物物理, 53, 250-253 (2013)

Mochizuki Y, Kohno F, Nishigaki K, Nemoto N., A pull-down method with a biotinylated bait protein prepared by cell-free translation using a puromycin linker. Anal. Biochem., 434, 93-95 (2013)

2012年

Ueno S, Kimura S, Ichiki T, Nemoto N., Improvement of a puromycin-linker to extend the selestion target varieties in cDNA display method. J. Biotechnol., 162, 299-302 (2012)

Ueno S, Yoshida S, Mondal A, Nishina K, Koyama M, Sakata I, Miura K, Hayashi Y, Nemoto N, Nishigaki K, Sakai T., In vitro selestion of a peptide antagonist of growth hormone secretagogue receptor using cDNA display. Proc. Natl. Acad. Sci. USA, 109, 11121-11126 (2012)

Nemoto N, Tsutsui C, Yamaguchi J, Ueno S, Machida M, Kobayashi T, Sakai T., Antagonistic effect of disulfide-rich peptide aptamers selected by cDNA display on inetrleukin-6-dependent cell proliferation. Biochem. Biophys. Res. Commun.. 421, 129-133 (2012)

Ueno S, Nemoto N., cDNA display: rapid stablilization of mRNA display. Methods in Mol. Biol., 805, 113-135 (2012)

Mochizuki Y, Nemoto N., Evolution of disulfide-rich peptide aptamers using cDNA display. Methods in Mol. Biol., 805, 237-250 (2012)

2011年

Mochizuki Y, Biyani M, Tsuji-Ueno S, Suzuki M, Nishigaki K, Husimi Y, Nemoto N., One-pot preparation of mRNA/cDNA display by a novel and versatile puromycin-linker DNA. ACS Comb. Sci., 13, 478-485 (2011)

Naimuddin M, Kobayashi S, Tsutsui C, Machida M, Nemoto N, Sakai T, Kubo T., Directed evolution of a three-finger neurotoxin by using cDNA display yields antagonists as well as agonists of interleukin-6 receptor signaling. Mol. Brain., 4, 2 (2011)

2009年

Yamaguchi J, Naimuddin M, Biyani M, Sasaki T, Machida M, Kubo T, Funatsu T, Husimi Y, Nemoto N., cDNA display: a novel screening method for functional disulfide-rich peptides by solid-phase synthesis and stabilization of mRNA-protein fusions. Nucleic Acids Res.. 16, e108 (2009)

1997年

Nemoto N, Miyamoto-Sato E, Husimi Y, Yanagawa H., In vitro virus: bonding of mRNA bearing puromycin at the 3´-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro. FEBS Lett. 414, 405-408 (1997)