- 論文
- 特許
- 2025年Moritani T, Masaki H, Yonehara R, Suzuki T, Arai H, Tsuchiya M, Nemoto N. A Novel Method for Preparing Uniform Micro-Sized Dry Powder Formulations, Including Aggregation-Controlled VHH. Antibodies (Basel). 2025 Mar 31;14(2):29.
- 2024年Munaweera TIK, Damnjanović J, Camagna M, Nezu M, Jia B, Hitomi K, Nemoto N, Nakano H. Substrate profiling of human transglutaminase 1 using cDNA display and next-generation sequencing. Biosci Biotechnol Biochem. 2024 May 22;88(6):620-629.
Aita T, Nemoto N. Mathematical consideration of massive estimation of dissociation rate constant for genotype-phenotype linking molecules bound to targets through washing/selection and next-generation sequencing. J Theor Biol. 2024 Dec 7;595:111944.
- 2023年Yonehara, R., Kumachi, S., Kashiwagi, K., Wakabayashi-Nakao, K., Motohashi, M., Murakami, T., Yanagisawa, T., Arai, H., Murakami, A., Ueno, Y., Nemoto, N., & Tsuchiya, M. A novel agonist with homobivalent single-domain antibodies that bind the FGF receptor 1 domain III functions as an FGF2 ligand. J Biol Chem. 2023 Feb;299(2):102804.
- 2022年Murakami T, Kumachi S, Matsunaga Y, Sato M, Wakabayashi-Nakao K, Masaki H, Yonehara R, Motohashi M, Nemoto N, Tsuchiya M., Construction of a Humanized Artificial VHH Library Reproducing Structural Features of Camelid VHHs for Therapeutics. Antibodies (Basel). 2022 Jan 30;11(1):10.
Damnjanović J, Odake N, Fan J, Camagna M, Jia B, Kojima T, Nemoto N, Hitomi K, Nakano H. Comprehensive analysis of transglutaminase substrate preference by cDNA display coupled with next-generation sequencing and bioinformatics. Sci Rep. 2022 Aug 9;12(1):13578.
- 2021年
Haga K, Takai-Todaka R, Matsumura Y, Song C, Takano T, Tojo T, Nagami A, Ishida Y, Masaki H, Tsuchiya M, Ebisudani T, Sugimoto S, Sato T, Yasuda H, Fukunaga K, Akihito Sawada A, Nemoto N, Murata K, Morimoto T, Katayama K., Nasal delivery of single-domain antibody improves symptoms of SARS-CoV-2 infection in an animal model. PLoS Pathog. 2021 Oct 14;17(10):e1009542.
Anzai H, Terai T, Wakabayashi-Nakao K, Noguchi T, Kumachi S, Tsuchiya M, Nemoto N., Interleukin-17A Peptide Aptamers with an Unexpected Binding Moiety Selected by cDNA Display under Heterogenous Conditions. ACS Med Chem Lett. 2021 Aug 27;12(9):1427-1434.
Jayathilake C, Nemoto N., cDNA Display-Mediated Immuno-PCR (cD-IPCR): An Ultrasensitive Immunoassay for Biomolecular Detection. Methods Mol Biol. 2021;2261:307-321.
- 2020年
Jayathilake C, Kumachi S, Arai H, Motohashi M, Terai T, Murakami A, Nemoto N., In vitro selection of anti-gliadin single-domain antibodies from a naïve library for cDNA-display mediated immuno-PCR. Anal Biochem. 2020 Jan 15:589:113490.
Arai H, Kumachi S, Nemoto N., cDNA Display: A Stable and Simple Genotype-Phenotype Coupling Using a Cell-Free Translation System. Methods Mol Biol. 2020:2070:43-56.
- 2019年Anzai H, Terai T, Jayathilake C, Suzuki T, Nemoto N., A novel immuno-PCR method using cDNA display. Anal Biochem. 2019 Aug 1:578:1-6.
- 2018年
Nemoto N, Kumachi S, Arai H., In vitro Selection of Single-Domain Antibody (VHH) using cDNA display.Methods Mol Biol. 2018:1827:269-285.
Suzuki T, Nochizuki Y, Kimura S, Akazawa-Ogawa Y, Hagihara Y, Nemoto N., Anti-survivin single-domain antibodies derived from an artificial library including three synthetic random regions by in vitro selection using cDNA display, Biochem Biophys Res Commun. 2018 Sep 10;503(3):2054-2060.
- 2017年
寺井琢也, 熊地重文, 根本直人, 「cDNAディスプレイ法によるペプチドスクリーニング技術」, 技術情報協会「ペプチド医薬品のスクリーニング・安定化・製剤化技術」第１章, 第６節, 40-49 (2017)
Kobayashi S, Terai T, Yoshikawa Y, Ohkawa R, Edihara M, Hayashi M, Takiguchi K, Nemoto N., In vitro selection of random peptides against artificial lipid bilayers: a potential tool to immobilize molecules on membranes, Chem Commun (Camb). 2017 Mar 25;53(24):3458-3461.
- 2016年
熊地重文, 根本直人, 「試験管内進化を加速するcDNA displayシステム」酵素工学ニュース, 76, 21-25 (2016)
根本直人, 「cDNAディスプレイによる機能性ペプチドアプタマーの創生」 日本生物工学会誌, 94, 481-484 (2016)
Kumachi S, Husimi Y, Nemoto N., An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display. ACS Omega. 2016 Jul 31;1(1):52-57.
- 2015年
Mochizuki Y, Suzuki T, Fujimoto K, Nemoto N., A Versatile puromycin-linker using cnvK for high-throughput in vitro selection by cDNA display. J Biotechnol. 2015 Oct 20:212:174-80.
Tanemura Y, Mochizuki Y, Kumachi S, Nemoto N., Easy and rapid binding assay for fanctional analysis of disulfide-containing peptides by a pull-down method using a puromycin-linker and a cell-free translation system. Biology (Basel). 2015 Mar 2;4(1):161-72.
- 2014年
Nemoto N, Fukushima T, Kumachi S, Suzuki M, Nishigaki K, kubo T., Versatile C-terminal specific biotinylation of proteins using both a puromycin-linker and a cell-free translation system for studying high-throughput protein-molecule interations. Anal Chem. 2014 Sep 2;86(17):8535-40.
Mochizuki Y, Nisigaki K, Nemoto N., Amino group binding peptide aptamers with double disulphide-bridged loops selected by in vitro selection using cDNA display. Chem Commun (Camb). 2014 May 30;50(42):5608-10.
- 2013年
根本直人, 「遺伝子型-表現型対応付け技術」 (株)エヌ・ティー・エス 『進化分子工学-高速分子進化によるタンパク質・核酸の開発-』第２編, 第３章, 161-170 (2013)
根本直人, 望月佑樹, 上野真吾 「cDNA displayによる分子デザイン -mRNA display(in vitro virus)からcDNA displayへ-」 生物物理, 53, 250-253 (2013)
Mochizuki Y, Kohno F, Nishigaki K, Nemoto N., A pull-down method with a biotinylated bait protein prepared by cell-free translation using a puromycin linker. Anal Biochem. 2013 Mar 1;434(1):93-5.
- 2012年
Ueno S, Kimura S, Ichiki T, Nemoto N., Improvement of a puromycin-linker to extend the selestion target varieties in cDNA display method. J Biotechnol. 2012 Dec 31;162(2-3):299-302.
Ueno S, Yoshida S, Mondal A, Nishina K, Koyama M, Sakata I, Miura K, Hayashi Y, Nemoto N, Nishigaki K, Sakai T., In vitro selestion of a peptide antagonist of growth hormone secretagogue receptor using cDNA display. Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11121-6.
Nemoto N, Tsutsui C, Yamaguchi J, Ueno S, Machida M, Kobayashi T, Sakai T., Antagonistic effect of disulfide-rich peptide aptamers selected by cDNA display on inetrleukin-6-dependent cell proliferation. Biochem Biophys Res Commun. 2012 Apr 27;421(1):129-33.
Ueno S, Nemoto N., cDNA display: rapid stablilization of mRNA display. Methods Mol Biol . 2012:805:113-35.
Mochizuki Y, Nemoto N., Evolution of disulfide-rich peptide aptamers using cDNA display. Methods Mol Biol. 2012:805:237-50.
- 2011年
Mochizuki Y, Biyani M, Tsuji-Ueno S, Suzuki M, Nishigaki K, Husimi Y, Nemoto N., One-pot preparation of mRNA/cDNA display by a novel and versatile puromycin-linker DNA. ACS Comb Sci. 2011 Sep 12;13(5):478-85.
Naimuddin M, Kobayashi S, Tsutsui C, Machida M, Nemoto N, Sakai T, Kubo T., Directed evolution of a three-finger neurotoxin by using cDNA display yields antagonists as well as agonists of interleukin-6 receptor signaling. Mol Brain. 2011 Jan 7:4:2.
- 2009年Yamaguchi J, Naimuddin M, Biyani M, Sasaki T, Machida M, Kubo T, Funatsu T, Husimi Y, Nemoto N., cDNA display: a novel screening method for functional disulfide-rich peptides by solid-phase synthesis and stabilization of mRNA-protein fusions. Nucleic Acids Res. 2009 Sep;37(16):e108.
- 1997年Nemoto N, Miyamoto-Sato E, Husimi Y, Yanagawa H., In vitro virus: bonding of mRNA bearing puromycin at the 3´-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro. FEBS Lett . 1997 Sep 8;414(2):405-8.