Publications/Patents

Publications

2019年

Anzai H, Terai T, Jayathilake C, Suzuki T, Nemoto N., A novel immuno-PCR method using cDNA display. Analytical Biochemistry, 578, 1-6 (2019)

2018年

Nemoto N, Kumachi S, Arai H., In vitro Selection of Single-Domain Antibody (VHH) using cDNA display, "Antibody Engineering 3rd Edition (edited by D. Nevoltis & P. Chames)", Methods in Mol. Biol., 1827, 269-285 (2018)

Suzuki T, Nochizuki Y, Kimura S, Akazawa-Ogawa Y, Hagihara Y, Nemoto N., Anti-survivin single-domain antibodies derived from an artificial library including three synthetic random regions by in vitro selection using cDNA display, Biochem. Biophys. Res. Commun., 503, 3, 2054-2060 (2018)

2017年

Kobayashi S, Terai T, Yoshikawa Y, Ohkawa R, Edihara M, Hayashi M, Takiguchi K, Nemoto N., In vitro selection of random peptides against artificial lipid bilayers: a potential tool to immobilize molecules on membranes, Chem. Commun. (Camb.), 53, 3458-3461 (2017)

2016年

Kumachi S, Husimi Y, Nemoto N., An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display. ACS Omega. 1, 52-57 (2016)

2015年

Mochizuki Y, Suzuki T, Fujimoto K, Nemoto N., A Versatile puromycin-linker using cnvK for high-throughput in vitro selection by cDNA display. J. Biotechnol. 212, 174-180 (2015)

Tanemura Y, Mochizuki Y, Kumachi S, Nemoto N., Easy and rapid binding assay for fanctional analysis of disulfide-containing peptides by a pull-down method using a puromycin-linker and a cell-free translation system, Biology (Basel), 4, 161-172 (2015)

2014年

Nemoto N, Fukushima T, Kumachi S, Suzuki M, Nishigaki K, kubo T., Versatile C-terminal specific biotinylation of proteins using both a puromycin-linker and a cell-free translation system for studying high-throughput protein-molecule interations, Anal. Chem., 86, 8535-8540 (2014)

Mochizuki Y, Nisigaki K, Nemoto N., Amino group binding peptide aptamers with double disulphide-bridged loops selected by in vitro selection using cDNA display., Chem. Commun. (Camb.), 50, 5608-5610 (2014)

2013年

Mochizuki Y, Kohno F, Nishigaki K, Nemoto N., A pull-down method with a biotinylated bait protein prepared by cell-free translation using a puromycin linker. Anal. Biochem., 434, 93-95 (2013)

2012年

Ueno S, Kimura S, Ichiki T, Nemoto N., Improvement of a puromycin-linker to extend the selestion target varieties in cDNA display method. J. Biotechnol., 162, 299-302 (2012)

Ueno S, Yoshida S, Mondal A, Nishina K, Koyama M, Sakata I, Miura K, Hayashi Y, Nemoto N, Nishigaki K, Sakai T., In vitro selestion of a peptide antagonist of growth hormone secretagogue receptor using cDNA display. Proc. Natl. Acad. Sci. USA, 109, 11121-11126 (2012)

Nemoto N, Tsutsui C, Yamaguchi J, Ueno S, Machida M, Kobayashi T, Sakai T., Antagonistic effect of disulfide-rich peptide aptamers selected by cDNA display on inetrleukin-6-dependent cell proliferation. Biochem. Biophys. Res. Commun.. 421, 129-133 (2012)

Ueno S, Nemoto N., cDNA display: rapid stablilization of mRNA display. Methods in Mol. Biol., 805, 113-135 (2012)

Mochizuki Y, Nemoto N., Evolution of disulfide-rich peptide aptamers using cDNA display. Methods in Mol. Biol., 805, 237-250 (2012)

2011年

Mochizuki Y, Biyani M, Tsuji-Ueno S, Suzuki M, Nishigaki K, Husimi Y, Nemoto N., One-pot preparation of mRNA/cDNA display by a novel and versatile puromycin-linker DNA. ACS Comb. Sci., 13, 478-485 (2011)

Naimuddin M, Kobayashi S, Tsutsui C, Machida M, Nemoto N, Sakai T, Kubo T., Directed evolution of a three-finger neurotoxin by using cDNA display yields antagonists as well as agonists of interleukin-6 receptor signaling. Mol. Brain., 4, 2 (2011)

2009年

Yamaguchi J, Naimuddin M, Biyani M, Sasaki T, Machida M, Kubo T, Funatsu T, Husimi Y, Nemoto N., cDNA display: a novel screening method for functional disulfide-rich peptides by solid-phase synthesis and stabilization of mRNA-protein fusions. Nucleic Acids Res.. 16, e108 (2009)

1997年

Nemoto N, Miyamoto-Sato E, Husimi Y, Yanagawa H., In vitro virus: bonding of mRNA bearing puromycin at the 3´-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro. FEBS Lett. 414, 405-408 (1997)